Anti-Phospho-p70 S6K (Thr398) Antibody

Product Name

Anti-Phospho-p70 S6K (Thr398) Antibody

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SKU/Catalog Number

P30407

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Phospho-p70 S6K (Thr398) Antibody (Catalog # P30407). Tested in WB applications. This antibody reacts with Drosophila.

Storage & Handling

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.

Cite This Product

Anti-Phospho-p70 S6K (Thr398) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P30407)

Host

Rabbit

Contents

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Clonality

Polyclonal

Clone Number

1B9

Isotype

IgG

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr398 of Drosophila p70 S6K protein, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Drosophila.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

P30407 is reactive to in Drosophila

Applications

P30407 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

55427 MW

Background of

p70 S6 kinase (p70 S6K) is activated in a signaling pathway that includes mTOR and is a mitogen-activated Ser/Thr protein kinase that is required for cell growth and G1 cell cycle progression (Xio et al., 2009). p70 S6K is controlled by multiple phosphorylation events located within the catalytic, linker and pseudosubstrate domains and subsequently phosphorylates specifically ribosomal protein S6 (Saitoh et al., 2002). Phosphorylation of Thr-229 in the catalytic domain and Thr-389 in the linker domain are most critical for kinase function. Inhibition of p70 activity inhibits the entry into S phase of the cell cycle and exhibits cell cycle arrest at G0/G1 phase, suggesting that the activation of p70 S6k plays an obligatory role in mediating mitogenic signals during cell activation (Xio et al., 2009).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000

Validation Images & Assay Conditions

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Western blot of Drosophila S2 cell lysate showing specific labeling of the ~70 kDa p70 S6K protein phosphorylated at Thr³⁹⁸ in the first lane (-). Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen in the second lane (+), but not by the corresponding non-phosphopeptide (not shown).

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